Data and figures are provided on the effects of fluoxetine, a selective serotin reuptake inhibitor (SSRI) antidepressant, on the gut microbiome and metabolome in a rat model to better understand the effect of this drug on women during gestation and lactation. Throughout pregnancy and lactation, female rats received the SSRI fluoxetine or vehicle. High resolution 16S ribosomal RNA marker gene sequencing and targeted metabolic analysis were used to assess the fecal microbiome and metabolic availability, respectively.

This dataset collected 40 ml of heparinized blood from 20 COVID-19 patients who were admitted to the University of Maryland Medical Center between June and August of 2020. Plasma samples were collected at a median of 10 or 7 days, dependent on the patient's risk group. Samples were analyzed using full-length recombinant protein in an enzyme-linked immunosorbent assay (ELISA). Antibodies were screened against 9 different SARS-CoV-2 proteins. Responses were observed against the spike (S) proteins, the receptor-binding domain (RBD), and the nucleocapsid (N) protein which were of the IgG1 and IgG3 subtypes. Studies using this dataset aim to identify the most relevant component of the anti-SARS-CoV-2 humoral immune response and enable researchers to design more meaningful immunomonitoring methods for anti-COVID19 vaccines.

This study investigates multiple vaccine strategies to enhance immunogenicity and protection against SARS-CoV-2 in aged mice. The study aims to determine the effect of a booster dose, with an emphasis on older age. Female three and eleven-month-old BALB/c mice were studied over the course of 38 weeks. Mice received BNT162b2 SARS-CoV-2 spike mRNA vaccine series, primary vaccination series, and mock Phosphate-buffered saline (PBS) injections. Single mouse experiments aimed to include 20 mice per group. Sample size and age criteria were chosen empirically based on the results of previous studies. Mice were randomly assigned to different treatment groups.

Researchers used a high-throughput sequencing-based assay to characterize the bacterial composition and diversity of 665 individual field-caught mosquitoes, as well as their species, genotype at an insecticide resistance locus, blood-meal composition, and the eukaryotic parasites and viruses they carry. This data was used to rigorously estimate the individual effect of each parameter on the bacterial diversity as well as the relative contribution of each parameter to the microbial composition.

Thirty-six-week-old again female C5BL/6 mice were injected with MSM for thirteen weeks. Researchers examined the effects of MSM on bone density and development using micro-computed tomographic images, serum analyses, and immunohistochemical analyses.

Nonarthritic anterior ischemic optic neuropathy (NAION) commonly causes sudden optic nerve (ON)-related vision loss. The rodent NAION model (rAION) closely resembles NAION in presentation and physiological responses. Researchers hypothesized that blocking pro-inflammatory prostaglandin (PGE2) production by inhibiting monoacylglycerol lipase or cyclooxygenase activity and co-administering PGJ2 would potentiate RGC survival following ischemic neuropathy. This study identified early rAION-associated optic nerve head (ONH) inflammatory gene expression responses and the anti-inflammatory prostaglandin PGJ2’s effects on those responses. Deep sequencing was performed on vehicle- and PGJ2-treated ONHs 3d post-rAION induction. Results were compared against responses from a retinal ischemia model. Animals were treated with PGJ2 and MAGL inhibitor KML29, or PGJ2 + COX inhibitor meloxicam. RGC survival was quantified by stereology.

Non-typhoidal Salmonella (NTS) is responsible for approximately 93 million cases of gastroenteritis annually. Little research has been directed towards a development of vaccines against Salmonella serogroups O:6,7 or O:8. Researchers constructed a live attenuated serogroup O:8 vaccine, CVS 1979, by deleting guaBA, htrA, and aroA from the genome of S. Newport. Over the course of 6 weeks, 6 to 8-week-old female BALB/c mice were immunized with live attenuated S. Newport vaccine CVD 1979 or sterile phosphate buffered saline (PBS) three times. Four weeks after the last immunization, mice were challenged intraperitoneally (i.p.) with wild-type Salmonella strains to assess the homologous and heterologous protection elicited by the live attenuated S. Newport vaccine CVD 1979.

Tissue from the optic nerve lamina region (ONLR), were dissected from both mice and humans. Immunohistochemistry (IHC) was then performed on tissue sections, and Ki67 mitosis assays assessed cell proliferation. Cell cultivation techniques isolated and cultured cells from ONLR and optic nerve tissues. Further experiments induced cell differentiation and neurosphere formation. Transgenic animal studies explored gene effects, while interventions included AAV-DTA construct injections and TAM/4OHT treatments. RNA isolation and qPCR analyzed gene expression, while imaging techniques visualized tissue morphology and axonal myelination.

The UMM Biorepository is a resource building effort that includes banking of blood samples from UMMS patients as well as collections of various biospecimens from collaborating UM researchers. State-of-the-art robotic freezer and liquidhandling equipment offer a secure and managed environment for biospecimen processing, storage and distribution. Data connected to the samples is obtained through the electronic health record and/or study-specific data collection, allowing for multi-disciplinary research that can impact a range of health issues. Access to data and biospecimens is dependent upon specific study requirements.

This dataset was generated to investigate long-lasting neurobiological adaptations to drugs of abuse. This dataset specifically supported the study of cocaine exposure impact of mitochondrial dynamics and morphology through early growth response factor 3 (Egr3) transcriptional regulation of mitochondria-related nuclear gene transcripts. Mitochondria-related nuclear genes were assayed following contingent or non-contingent cocaine exposure in rodents and were also examined in postmortem nucleus accumbens (NAc) of cocaine dependents.

Supplemental data in tabular forms include human demographics, DNA sequencing primers, mitochondrial statistical analysis, and gene regulations.

The following data elements were observed over a period of 7-10 days: Egr3 change in response to cocaine vs saline between rats and human postmortem issue; Egr3 binding changes in various genes promoting mitochondria in response to cocaine vs saline between rats and human postmortem issue; mRNA change in response to cocaine and saline between rats and human postmortem issue and between cocaine addict and control group; mRNA change in response to cocaine and saline between rats and human dead issue; Ribosome (the RNA binding proteins) associated mRNA changes in dopamine receptor-1 containing medium spiny neurons (D1 MSNs) and D2 MSNs in response to cocaine and saline between cocaine addict and control group.