Find Datasets from
UMB Researchers
of 2 Next >
Results Found: 14
  • Antidepressant treatment with fluoxetine during pregnancy and lactation modulates the gut microbiome and metabolome in a rat model relevant to depression
    UMB Dataset

    Authors
    Anouschka S. Ramsteijn
    Eldin Jašarević
    Danielle J. Houwing
    Tracy L. Bale
    1 more author(s)...
    Description

    Data and figures are provided on the effects of fluoxetine, a selective serotin reuptake inhibitor (SSRI) antidepressant, on the gut microbiome and metabolome in a rat model to better understand the effect of this drug on women during gestation and lactation. Throughout pregnancy and lactation, female rats received the SSRI fluoxetine or vehicle. High resolution 16S ribosomal RNA marker gene sequencing and targeted metabolic analysis were used to assess the fecal microbiome and metabolic availability, respectively.

    Subject
    Antidepressive Agents
    Lactation
    Pregnancy
    Geographic Coverage
    International
    Access Rights
    Unrestricted access
  • Deep dissection of the antiviral immune profile of patients with COVID-19
    UMB Dataset

    Authors
    Djordje Atanackovic
    Stephanie V. Avila
    Forat Lutfi
    Diego de Miguel-Perez
    16 more author(s)...
    Description

    This dataset collected 40 ml of heparinized blood from 20 COVID-19 patients who were admitted to the University of Maryland Medical Center between June and August of 2020. Plasma samples were collected at a median of 10 or 7 days, dependent on the patient's risk group. Samples were analyzed using full-length recombinant protein in an enzyme-linked immunosorbent assay (ELISA). Antibodies were screened against 9 different SARS-CoV-2 proteins. Responses were observed against the spike (S) proteins, the receptor-binding domain (RBD), and the nucleocapsid (N) protein which were of the IgG1 and IgG3 subtypes. Studies using this dataset aim to identify the most relevant component of the anti-SARS-CoV-2 humoral immune response and enable researchers to design more meaningful immunomonitoring methods for anti-COVID19 vaccines.

    Subject
    Antibodies, Neutralizing/immunology
    Antibodies, Viral/immunology
    COVID-19/immunology
    Viral Proteins/immunology
    Geographic Coverage
    Baltimore (Md.)
    Timeframe
    2020
    Access Rights
    Unrestricted access
  • mRNA booster vaccination protects aged mice against the SARS-CoV-2 Omicron variant
    UMB Dataset

    Authors
    Etsuro Nanishi
    Marisa E. McGrath
    Timothy R. O’Meara
    Soumik Barman
    17 more author(s)...
    Description

    This study investigates multiple vaccine strategies to enhance immunogenicity and protection against SARS-CoV-2 in aged mice. The study aims to determine the effect of a booster dose, with an emphasis on older age. Female three and eleven-month-old BALB/c mice were studied over the course of 38 weeks. Mice received BNT162b2 SARS-CoV-2 spike mRNA vaccine series, primary vaccination series, and mock Phosphate-buffered saline (PBS) injections. Single mouse experiments aimed to include 20 mice per group. Sample size and age criteria were chosen empirically based on the results of previous studies. Mice were randomly assigned to different treatment groups.

    Subject
    COVID-19/prevention & control
    Viral Vaccines/genetics
    Access Rights
    Unrestricted access
    Local Experts
    Matthew B. Frieman
  • Relative contributions of various endogenous and exogenous factors to the mosquito microbiota
    UMB Dataset

    Authors
    Haikel N. Bogale
    Matthew V. Cannon
    Kalil Keita
    Denka Camara
    10 more author(s)...
    Description

    Researchers used a high-throughput sequencing-based assay to characterize the bacterial composition and diversity of 665 individual field-caught mosquitoes, as well as their species, genotype at an insecticide resistance locus, blood-meal composition, and the eukaryotic parasites and viruses they carry. This data was used to rigorously estimate the individual effect of each parameter on the bacterial diversity as well as the relative contribution of each parameter to the microbial composition.

    Subject
    Pest Control, Biological
    Access Rights
    Unrestricted access
  • Methylsulfonylmethane Increases the Alveolar Bone Density of Mandibles in Aging Female Mice
    UMB Dataset

    Authors
    Hanan Aljohani
    Linda T. Senbanjo
    Mohammed Al Qranei
    Joseph P. Stains
    1 more author(s)...
    Description

    Thirty-six-week-old again female C5BL/6 mice were injected with MSM for thirteen weeks. Researchers examined the effects of MSM on bone density and development using micro-computed tomographic images, serum analyses, and immunohistochemical analyses.

    Subject
    Osteogenesis
    Access Rights
    Unrestricted access
  • Mechanoactivation of NOX2-generated ROS elicits persistent TRPM8 Ca2+ signals that are inhibited by oncogenic KRas
    UMB Dataset

    Authors
    Stephen J. Pratt
    Rachel M. Lee
    Cakarina T. Chang
    Erick O. Hernández-Ochoa
    11 more author(s)...
    Description

    Human breast MCF10A epithelial cells were subjected to mechanical stimulation using glass pipettes on substrates with varying stiffness. Data on NOX2 or TRPM8 mRNA expression and its correlation with breast cancer patient outcomes were collected from the Kaplan–Meier Plotter database. Statistical analyses were performed using MATLAB.

    Subject
    Breast/pathology
    Access Rights
    Unrestricted access
  • Approaches to Potentiated Neuroprotective Treatment in the Rodent Model of Ischemic Optic Neuropathy
    UMB Dataset

    Authors
    Zara Mehrabian
    Yan Guo
    Neil R. Miller
    Amanda D. Henderson
    2 more author(s)...
    Description

    Nonarthritic anterior ischemic optic neuropathy (NAION) commonly causes sudden optic nerve (ON)-related vision loss. The rodent NAION model (rAION) closely resembles NAION in presentation and physiological responses. Researchers hypothesized that blocking pro-inflammatory prostaglandin (PGE2) production by inhibiting monoacylglycerol lipase or cyclooxygenase activity and co-administering PGJ2 would potentiate RGC survival following ischemic neuropathy. This study identified early rAION-associated optic nerve head (ONH) inflammatory gene expression responses and the anti-inflammatory prostaglandin PGJ2’s effects on those responses. Deep sequencing was performed on vehicle- and PGJ2-treated ONHs 3d post-rAION induction. Results were compared against responses from a retinal ischemia model. Animals were treated with PGJ2 and MAGL inhibitor KML29, or PGJ2 + COX inhibitor meloxicam. RGC survival was quantified by stereology.

    Subject
    Optic Nerve Diseases
    Timeframe
    2021
    Access Rights
    Unrestricted access
  • Refinement of a Live Attenuated Salmonella enterica Serovat Newport Vaccine with Improved Safety
    UMB Dataset

    Description

    Non-typhoidal Salmonella (NTS) is responsible for approximately 93 million cases of gastroenteritis annually. Little research has been directed towards a development of vaccines against Salmonella serogroups O:6,7 or O:8. Researchers constructed a live attenuated serogroup O:8 vaccine, CVS 1979, by deleting guaBA, htrA, and aroA from the genome of S. Newport. Over the course of 6 weeks, 6 to 8-week-old female BALB/c mice were immunized with live attenuated S. Newport vaccine CVD 1979 or sterile phosphate buffered saline (PBS) three times. Four weeks after the last immunization, mice were challenged intraperitoneally (i.p.) with wild-type Salmonella strains to assess the homologous and heterologous protection elicited by the live attenuated S. Newport vaccine CVD 1979.

    Subject
    Salmonella Vaccines
    Vaccine Development
    Access Rights
    Unrestricted access
  • The optic nerve lamina region is a neural progenitor cell niche
    UMB Dataset

    Authors
    Steven L. Bernstein
    Yan Guo
    Rebecca Fawcett
    Candace Kerr
    3 more author(s)...
    Description

    Tissue from the optic nerve lamina region (ONLR), were dissected from both mice and humans. Immunohistochemistry (IHC) was then performed on tissue sections, and Ki67 mitosis assays assessed cell proliferation. Cell cultivation techniques isolated and cultured cells from ONLR and optic nerve tissues. Further experiments induced cell differentiation and neurosphere formation. Transgenic animal studies explored gene effects, while interventions included AAV-DTA construct injections and TAM/4OHT treatments. RNA isolation and qPCR analyzed gene expression, while imaging techniques visualized tissue morphology and axonal myelination.

    Subject
    Optic Nerve
    Access Rights
    Unrestricted access
    Approval required
  • University of Maryland Medicine Biorepository
    UMB Dataset

    Alternate Title(s)
    UMM Biorepository, University of Maryland Genetics BIObank
    Description

    The UMM Biorepository is a resource building effort that includes banking of blood samples from UMMS patients as well as collections of various biospecimens from collaborating UM researchers. State-of-the-art robotic freezer and liquidhandling equipment offer a secure and managed environment for biospecimen processing, storage and distribution. Data connected to the samples is obtained through the electronic health record and/or study-specific data collection, allowing for multi-disciplinary research that can impact a range of health issues. Access to data and biospecimens is dependent upon specific study requirements.

    Subject
    Biological Specimen Banks
    Access Rights
    Approval required
    University of Maryland personnel only
    Local Experts
    Alan R. Shuldiner
    Coleen M. Damcott