UMB Data Catalog

A project of the Health Sciences and Human Services Library
UMB Dataset

Mechanoactivation of NOX2-generated ROS elicits persistent TRPM8 Ca2+ signals that are inhibited by oncogenic KRas

UID: 220
Author(s): Stephen J. Pratt*, Rachel M. Lee, Cakarina T. Chang, Erick O. Hernández-Ochoa, David A. Annis...
* Corresponding Author
Description
Human breast MCF10A epithelial cells were subjected to mechanical stimulation using glass pipettes on substrates with varying stiffness. Data on NOX2 or TRPM8 mRNA expression and its correlation with breast cancer patient outcomes were collected from the Kaplan–Meier Plotter database. Statistical analyses were performed using MATLAB.
Subject Domain
Breast/pathology
Keywords
Cell Transformation, Neoplastic
Mechanotransduction, Cellular

Access

Restrictions
Unrestricted access
Instructions
There are no restrictions on the availability of materials or information. Data are provided in this manuscript as well as in Dataset S1. All MATLAB analysis code used in this manuscript is publicly available in a GitHub repository archived on Zenodo.
Access via Zenodo

mSLED analysis code
Accession #: 10.5281/zenodo.3986143

Access via Supplementary information

Dataset S1

Associated Publications
Pratt SJP, Lee RM, Chang KT, Hernández-Ochoa EO, Annis DA, Ory EC, Thompson KN, Bailey PC, Mathias TJ, Ju JA, Vitolo MI, Schneider MF, Stains JP, Ward CW, Martin SS. Mechanoactivation of NOX2-generated ROS elicits persistent TRPM8 Ca2+ signals that are inhibited by oncogenic KRas. Proc Natl Acad Sci U S A. 2020 Oct 20;117(42):26008-26019.
Data Type
Biospecimens
Experimental
Equipment Used
Molecular Devices FlexStation 3 multi-mode microplate reader

A versatile device for performing various assays and measurements in microplate formats.

Olympus FV1000 confocal laser

An advanced imaging system capable of capturing detailed three-dimensional images with high precision.

Olympus IX81 microscope

A sophisticated imaging tool designed for high-resolution observation of biological samples.

Sutter Instrument MP-225 motorized micromanipulator

A precision instrument for manipulating microscale objects with motorized control.

Software Used
MATLAB

A high-level programming language and environment for numerical computing and visualization.

Study Type
Interventional
Dataset Format(s)
XLS
Data Collection Instruments
One-Way ANOVA
Hertzian contact mechanics equation
Tukey's Honest Significant Difference (HSD) criterion
Dataset Size
2.30MB
Grant Support
Cigarette Restitution Fund Program/Maryland. Department of Health
BX002746/US Department of Veterans Affairs
METAvivor Foundation/METAvivor Foundation
RSG-18-028-01-CSM/American Cancer Society.
133192-PF-19-017-01-CSM/American Cancer Society
FA9550-16-1-0052/Air Force Office of Scientific Research (U.S.)
R01-CA124704/National Institutes of Health (U.S.)
5T32GM008181-30/National Institutes of Health (U.S.)
1F31CA232393-01/National Institutes of Health (U.S.)
T32CA154274/National Institutes of Health (U.S.)
5T32CA154274-07/National Institutes of Health (U.S.)
K01CA166576/National Institutes of Health (U.S.)
R37-AR055099/National Institutes of Health (U.S.)
P30-CA134274/National Cancer Institute (U.S.)