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Mechanoactivation of NOX2-generated ROS elicits persistent TRPM8 Ca2+ signals that are inhibited by oncogenic KRas
UMB Dataset

UID: 220

Author(s): Stephen J. Pratt*, Rachel M. Lee, Cakarina T. Chang, Erick O. Hernández-Ochoa, David A. Annis, Eleanor C. Ory, Keyata N. Thompson, Patrick C. Bailey, Trevor J. Mathias, Julia A. Ju, Michele I. Vitolo, Martin F. Schneider, Joseph P. Stains, Christopher W. Ward, Stuart S. Martin* * Corresponding Author
Description
Human breast MCF10A epithelial cells were subjected to mechanical stimulation using glass pipettes on substrates with varying stiffness. Data on NOX2 or TRPM8 mRNA expression and its correlation with breast cancer patient outcomes were collected from the Kaplan–Meier Plotter database. Statistical analyses were performed using MATLAB.
Subject Domain
Keywords
Access via Zenodo

mSLED analysis code
Accession #: 10.5281/zenodo.3986143

Access Restrictions
Unrestricted access
Access Instructions
There are no restrictions on the availability of materials or information. Data are provided in this manuscript as well as in Dataset S1. All MATLAB analysis code used in this manuscript is publicly available in a GitHub repository archived on Zenodo.
Associated Publications
Data Type
Equipment Used
Molecular Devices FlexStation 3 multi-mode microplate reader
Olympus FV1000 confocal laser
Olympus IX81 microscope
Sutter Instrument MP-225 motorized micromanipulator
Software Used
MATLAB
Study Type
Interventional
Dataset Format(s)
XLS
Data Collection Instruments
One-Way ANOVA
Hertzian contact mechanics equation
Tukey's Honest Significant Difference (HSD) criterion
Dataset Size
2.30MB
Grant Support
Cigarette Restitution Fund Program/Maryland. Department of Health
BX002746/US Department of Veterans Affairs
METAvivor Foundation/METAvivor Foundation
RSG-18-028-01-CSM/American Cancer Society.
133192-PF-19-017-01-CSM/American Cancer Society
FA9550-16-1-0052/Air Force Office of Scientific Research (U.S.)
P30-CA134274/National Cancer Institute (U.S.)