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Refinement of a Live Attenuated Salmonella enterica Serovat Newport Vaccine with Improved Safety

UID: 207
Description
Non-typhoidal Salmonella (NTS) is responsible for approximately 93 million cases of gastroenteritis annually. Little research has been directed towards a development of vaccines against Salmonella serogroups O:6,7 or O:8. Researchers constructed a live attenuated serogroup O:8 vaccine, CVS 1979, by deleting guaBA, htrA, and aroA from the genome of S. Newport. Over the course of 6 weeks, 6 to 8-week-old female BALB/c mice were immunized with live attenuated S. Newport vaccine CVD 1979 or sterile phosphate buffered saline (PBS) three times. Four weeks after the last immunization, mice were challenged intraperitoneally (i.p.) with wild-type Salmonella strains to assess the homologous and heterologous protection elicited by the live attenuated S. Newport vaccine CVD 1979.
Subject of Study
Subject Domain
Subject Gender
Female
Keywords

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Restrictions
Unrestricted access
Instructions
The data presented in this study are contained within the article and supplementary material.
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datatables

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Unmodified Western bots

Associated Publications
Data Type
Equipment Used
Bio-Rad Trans-Blot Turbo Transfer System
ChemiDoc MP Imaging System
Trans-Blot Electrophoretic Cell System
Software Used
GraphPad Prism 7.0
Image Lab 5.1
Study Type
Interventional
Dataset Format(s)
PDF
Dataset Size
161kb
Grant Support
U19 AI109776-01/National Institute of Allergy and Infectious Diseases (U.S.)
5T32DK067872-15/National Institute of Health (U.S.)