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UMB Dataset

The optic nerve lamina region is a neural progenitor cell niche

UID: 216
Author(s): Steven L. Bernstein*, Yan Guo, Rebecca Fawcett, Candace Kerr, Jeffrey Stern...
* Corresponding Author
Description
Tissue from the optic nerve lamina region (ONLR), were dissected from both mice and humans. Immunohistochemistry (IHC) was then performed on tissue sections, and Ki67 mitosis assays assessed cell proliferation. Cell cultivation techniques isolated and cultured cells from ONLR and optic nerve tissues. Further experiments induced cell differentiation and neurosphere formation. Transgenic animal studies explored gene effects, while interventions included AAV-DTA construct injections and TAM/4OHT treatments. RNA isolation and qPCR analyzed gene expression, while imaging techniques visualized tissue morphology and axonal myelination.
Subject of Study
Subject Domain
Optic Nerve
Keywords
Cell Differentiation
Neural Stem Cells

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Restrictions
Unrestricted access
Approval required
Instructions
Data have been deposited in Figshare. ONLR-NPC cell culture aliquots will be provided on request along with the appropriate institutional approvals (IRB/IACUC) and materials handling fee.
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Images
Accession #: 10.6084/m9.figshare.12250223

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Images
Accession #: 10.6084/m9.figshare.12250232

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Images
Accession #: 10.6084/m9.figshare.12250241

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Images
Accession #: 10.6084/m9.figshare.12251945

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Images
Accession #: 10.6084/m9.figshare.12251957

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ONLR-NPC cell culture aliquots

Associated Publications
Bernstein SL, Guo Y, Fawcett RJ, Stern JH, Temple S, Mehrabian Z. The optic nerve lamina region is a neural progenitor cell niche. Bio. Sci. 2020 Aug 11;117(32):19287-19298.
Data Type
Genetic
Biospecimens
Imaging
Equipment Used
iCycler

A thermal cycler instrument used for performing PCR (polymerase chain reaction) reactions, allowing for precise temperature control during DNA amplification.

Invitrogen SuperScript First-Strand synthesis system

A molecular biology kit used for reverse transcription of RNA into complementary DNA (cDNA), enabling the study of gene expression through quantitative PCR (qPCR) analysis.

iQ SYBR Green Supermix

A ready-to-use reagent for qPCR assays, containing all necessary components for efficient DNA amplification and fluorescence detection using SYBR Green dye.

Nikon flourescent microscope

An advanced imaging system equipped with fluorescence capabilities, allowing for the visualization of fluorescently labeled cells and tissues.

Olympus E900 microscope

A high-quality optical microscope used for examining tissue samples and cellular structures with precision and clarity.

Qiagen RNeasy micro kit

A molecular biology kit used for the isolation and purification of high-quality RNA from biological samples, enabling downstream applications such as gene expression analysis by qPCR.

Thermo Scientific Tecnai transmission electron microscope

An electron microscope used for ultrastructural analysis of biological samples, providing detailed images of cellular organelles and structures at nanometer resolution.

Zeiss two-photon microscope

A cutting-edge imaging instrument that utilizes two-photon excitation to provide high-resolution, three-dimensional images of biological samples, particularly suited for deep tissue imaging.

Study Type
Observational
Dataset Format(s)
TIFF
Grant Support
R01 EY015304/National Institute of Health (U.S.)
S10 RR026870/National Institute of Health (U.S.)