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The optic nerve lamina region is a neural progenitor cell niche
UMB Dataset

UID: 216

Author(s): Steven L. Bernstein*, Yan Guo, Rebecca Fawcett, Candace Kerr, Jeffrey Stern, Sally Temple, Zara Mehrabian * Corresponding Author
Tissue from the optic nerve lamina region (ONLR), were dissected from both mice and humans. Immunohistochemistry (IHC) was then performed on tissue sections, and Ki67 mitosis assays assessed cell proliferation. Cell cultivation techniques isolated and cultured cells from ONLR and optic nerve tissues. Further experiments induced cell differentiation and neurosphere formation. Transgenic animal studies explored gene effects, while interventions included AAV-DTA construct injections and TAM/4OHT treatments. RNA isolation and qPCR analyzed gene expression, while imaging techniques visualized tissue morphology and axonal myelination.
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Accession #: 10.6084/m9.figshare.12250223

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Accession #: 10.6084/m9.figshare.12250232

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Accession #: 10.6084/m9.figshare.12250241

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Accession #: 10.6084/m9.figshare.12251945

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Accession #: 10.6084/m9.figshare.12251957

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ONLR-NPC cell culture aliquots

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Data have been deposited in Figshare. ONLR-NPC cell culture aliquots will be provided on request along with the appropriate institutional approvals (IRB/IACUC) and materials handling fee.
Associated Publications
Data Type
Equipment Used
Invitrogen SuperScript First-Strand synthesis system
iQ SYBR Green Supermix
Nikon flourescent microscope
Olympus E900 microscope
Qiagen RNeasy micro kit
Thermo Scientific Tecnai transmission electron microscope
Zeiss two-photon microscope
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