mRNA booster vaccination protects aged mice against the SARS-CoV-2 Omicron variant

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UMB Dataset

mRNA booster vaccination protects aged mice against the SARS-CoV-2 Omicron variant

UID: 189

Author(s): Etsuro Nanishi, Marisa E. McGrath, Timothy R. O’Meara, Soumik Barman, Jingyou Yu... See more...

Huahua Wan, Carly A. Dillen, Manisha Menon, Hyuk-Soo Seo, Kijun Song, Andrew Z. Xu, Luke Sebastian, Byron Brook, Anna-Nicole Bosco, Francesco Borriello, Robert K. Ernst, Dan H. Barouch, Sirano Dhe-Paganon, Ofer Levy, Matthew B. Frieman, David J. Dowling*

* Corresponding Author

Description: This study investigates multiple vaccine strategies to enhance immunogenicity and protection against SARS-CoV-2 in aged mice. The study aims to determine the effect of a booster dose, with an emphasis on older age. Female three and eleven-month-old BALB/c mice were studied over the course of 38 weeks. Mice received BNT162b2 SARS-CoV-2 spike mRNA vaccine series, primary vaccination series, and mock Phosphate-buffered saline (PBS) injections. Single mouse experiments aimed to include 20 mice per group. Sample size and age criteria were chosen empirically based on the results of previous studies. Mice were randomly assigned to different treatment groups.
Local Experts: Matthew B. Frieman

Subject of Study

Subject Domain

COVID-19/prevention & control

Viral Vaccines/genetics

Subject Sex

Female

Keywords

Antibodies, Viral

Access

Restrictions: Unrestricted access
Instructions: All data supporting the findings of this study are available within the supplementary information files (Supplementary Data 1). The data corresponds to the figures that are in the article.; Access via Supplementary Data 1

Associated Publications

Nanishi, E., McGrath, M. E., O'Meara, T. R., Barman, S., Yu, J., Wan, H., Dillen, C. A., Menon, M., Seo, H. S., Song, K., Xu, A. Z., Sebastian, L., Brook, B., Bosco, A. N., Borriello, F., Ernst, R. K., Barouch, D. H., Dhe-Paganon, S., Levy, O., Frieman, M. B., & Dowling, D. J. (2022). mRNA booster vaccination protects aged mice against the SARS-CoV-2 Omicron variant. Communications biology, 5(1), 790. https://doi.org/10.1038/s42003-022-03765-3

Data Type

Equipment Used

BD LSR II Flow Cytometer

Bead ruptor (Omni International Inc.)

Costar 96-well cell culture multiple well plate, flat bottom, with lid

UltraPure agarose (Invitrogen)

Software Used

FlowJo v.10.8.1

Study Type

Interventional

Longitudinal

Dataset Format(s)

XLSX

Dataset Size

50 KB

Grant Support: HHSN272201400052C/United States. National Institute of Allergy and Infectious Diseases

75N93019C00044/United States. National Institute of Allergy and Infectious Diseases

HHSN272201800047C/United States. National Institute of Allergy and Infectious Diseases

1R21AI137932-01A1/National Institutes of Health (U.S.)

ASPR-20-01495/Biomedical Advanced Research and Development Authority (U.S.)