UMB Data Catalog

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Description
This study analyzed samples from 73 healthy donors and 81 individuals with HIV, divided into Natural Viral Suppressors (NVS), untreated non-EC patients (UP), and treated patients (TP). Samples were collected from various sources and subjected to serological, phenotypic, and virological assessments. Peripheral blood mononuclear cells (PBMCs) were isolated, CD4+T cells were stimulated, and flow cytometry was used to measure CCR5 and CXCR4 expression. Viral stocks were prepared from infectious molecular clones (IMCs), and virus growth kinetics were assessed. Immunophenotypic studies, cytokine quantification, and gene expression analyses were conducted.
Subject of Study
Subject Domain
Keywords

Access

Restrictions
Unrestricted access
Instructions
Source data for the main figures in the manuscript can be accessed at figshare.
Access via figshare

Source data for the main figures
Accession #: 10.6084/m9.figshare.25125356.v4

Associated Publications
Data Type
Equipment Used
Cytek Aurora Flow Cytometer
Promega FuGENE6 Transfection Reagent
Qiagen RNeasy RNA Extraction Columns
Quanterix SIMOA Cytokine Assay Kit
Roche LC480 Thermocycler
Software Used
Cytobank
Study Type
Observational
Dataset Format(s)
XLSX
Dataset Size
208.48 kB
Grant Support