UMB Data Catalog

A project of the Health Sciences and Human Services Library
Find Datasets from
UMB Researchers
Include your dataset

IFNa induces CCR5 in CD4+ T cells of HIV patients causing pathogenic elevation
UMB Dataset

UID: 226

Author(s): Hélène Le Buanec, Valérie Schiavon, Marine Merandet, Alexandre How-Kit, Hongshuo Song, David Bergerat, Céline Fombellida-Lopez, Armand Bensussan, Jean-David Bouaziz, Arsène Burny, Gilles Darcis, Mohammad M. Sajadi, Shyamasundaran Kottilil, Daniel Zagury, Robert C. Gallo* * Corresponding Author
Description
This study analyzed samples from 73 healthy donors and 81 individuals with HIV, divided into Natural Viral Suppressors (NVS), untreated non-EC patients (UP), and treated patients (TP). Samples were collected from various sources and subjected to serological, phenotypic, and virological assessments. Peripheral blood mononuclear cells (PBMCs) were isolated, CD4+T cells were stimulated, and flow cytometry was used to measure CCR5 and CXCR4 expression. Viral stocks were prepared from infectious molecular clones (IMCs), and virus growth kinetics were assessed. Immunophenotypic studies, cytokine quantification, and gene expression analyses were conducted.
Subject of Study
Human
Subject Domain
HIV Infections/therapy
Keywords
Antiretroviral Therapy
CD4-Positive T-Lymphocytes
Interferon-alpha
Access via figshare

Source data for the main figures
Accession #: 10.6084/m9.figshare.25125356.v4

Access Restrictions
Unrestricted access
Access Instructions
Source data for the main figures in the manuscript can be accessed at figshare.
Associated Publications
Le Buanec H, Schiavon V, Merandet M, How-Kit A, Song H, Bergerat D, Fombellida-Lopez C, Bensussan A, Bouaziz JD, Burny A, Darcis G, Sajadi MM, Kottilil S, Zagury D, Gallo RC. IFNa induces CCR5 in CD4+ T cells of HIV patients causing pathogenic elevation. Commun Med (Lond). 2024 Mar 19;4(1):52.
Data Type
Biospecimens
Equipment Used
Cytek Aurora Flow Cytometer

a high-performance instrument for analyzing cell populations and biomolecules.

Promega FuGENE6 Transfection Reagent

facilitates efficient delivery of nucleic acids into cells for transfection experiments.

Qiagen RNeasy RNA Extraction Columns

a reliable tool for isolating high-quality RNA from various sources.

Quanterix SIMOA Cytokine Assay Kit

highly sensitive immunoassays used for quantifying cytokine levels in biological samples.

Roche LC480 Thermocycler

a versatile machine used for precise temperature control in PCR reactions.

Software Used
Cytobank

Cloud-based data analysis platform.

Study Type
Observational
Dataset Format(s)
XLSX
Dataset Size
208.48 kB