UMB Data Catalog

A project of the Health Sciences and Human Services Library
UMB Dataset

Relative contributions of various endogenous and exogenous factors to the mosquito microbiota

UID: 211
Author(s): Haikel N. Bogale, Matthew V. Cannon, Kalil Keita, Denka Camara, Yaya Barry...
* Corresponding Author
Description
Researchers used a high-throughput sequencing-based assay to characterize the bacterial composition and diversity of 665 individual field-caught mosquitoes, as well as their species, genotype at an insecticide resistance locus, blood-meal composition, and the eukaryotic parasites and viruses they carry. This data was used to rigorously estimate the individual effect of each parameter on the bacterial diversity as well as the relative contribution of each parameter to the microbial composition.
Timeframe
2016 - 2017
Geographic Coverage
Guinea
Mali
Subject of Study
Anopheles
Subject Domain
Pest Control, Biological
Keywords
Anopheles
Entomology
Mosquito Vectors/microbiology
Mosquito-Borne Diseases/prevention & control

Access

Restrictions
Unrestricted access
Instructions
Data generated or analyzed during this study are included in this published article and its additional files. All raw sequences are available in the NCBI Sequence Read Archive. The custom scripts supporting the conclusions of this article are available in the MosqMicrobiome_Paper repository on GitHub.
Access via Article

generated data

Access via NCBI SRA

raw sequences
Accession #: PRJNA663576

Access via GitHub

custom scripts

Associated Publications
Bogale HN, Cannon MV, Keita K, Camara D, Barry Y, Keita M, Coulibaly D, Kone AK, Doumbo OK, Thera MA, Plowe CV, Travassos M, Irish S, Serre D. Relative contributions of various endogenous and exogenous factors to the misquito microbiota. Parasite Vectors. 2020 Dec 10; 13(619).
Data Type
Biospecimens
Genomic
Equipment Used
GoTaq DNA Polymerase

A proprietary formulation of Taq polymerase that gives robust amplification equal to and, in some cases, superior to that of standard Taq.

Illumina HiSeq 2500 System

High throughput DNA and RNA sequencing system

M-MLV Reverse Transcriptase

RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates.

NanoDrop spectrophotometer

Full-spectrum, UV-Vis spectrophotometer that quantifies and assesses purity of DNA, RNA, Protein and more

Qiagen DNeasy kit

A kit used for efficient extraction of DNA from various sample types.

TissueLyser

Used for sample management

Software Used
BLAST

Algorithm and program for comparing primary biological sequence information, such as the amino-acid sequences of proteins or the nucleotides of DNA and/or RNA sequences.

DADA2

Open source software R package for modeling and correcting Illumina sequenced amplicon errors. Fast and accurate sample inference from amplicon data with single nucleotide resolution.

FLASh

An accurate and fast tool to merge paired-end reads that were generated from DNA fragments whose lengths are shorter than twice the length of reads.

phyloseq

Software for handling and analysis of high-throughput microbiome census data.

Study Type
Observational
Dataset Format(s)
XLS, FASTQ, TIFF, RMD
Data Collection Instruments
Principal Coordinates Analysis
Bray–Curtis dissimilarity
Adonis function