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  • Stereolithography Code File for Digital Design and Fabrication of a Universal Rat Intraoral Device
    UMB Dataset

    Authors
    Radi Masri
    Mary Ann Jabra-Rizk
    Description

    Denture stomatitis (DS) is a chronic oral infection prevalent in denture wearers. In order to study the disease and evaluate therapeutic strategies, an intraoral dental appliance for use in a rat animal model, with universal fit, was created in the 3Shape Dental Design System. This code file was then generated for fabricating the device via 3D printing. STL format is supported by any software packages used for computer-aided design (CAD), 3D printing, rapid prototyping, and computer-aided manufacturing (CAM).

    Subject
    Equipment Design
    Stereolithography
    Access Rights
    Unrestricted access
  • Antidepressant treatment with fluoxetine during pregnancy and lactation modulates the gut microbiome and metabolome in a rat model relevant to depression
    UMB Dataset

    Authors
    Anouschka S. Ramsteijn
    Eldin Jašarević
    Danielle J. Houwing
    Tracy L. Bale
    1 more author(s)...
    Description

    Data and figures are provided on the effects of fluoxetine, a selective serotin reuptake inhibitor (SSRI) antidepressant, on the gut microbiome and metabolome in a rat model to better understand the effect of this drug on women during gestation and lactation. Throughout pregnancy and lactation, female rats received the SSRI fluoxetine or vehicle. High resolution 16S ribosomal RNA marker gene sequencing and targeted metabolic analysis were used to assess the fecal microbiome and metabolic availability, respectively.

    Subject
    Antidepressive Agents
    Lactation
    Pregnancy
    Geographic Coverage
    International
    Access Rights
    Unrestricted access
  • Sex Differences in the Expression of Calcitonin Gene-Related Peptide Receptor Components in the Spinal Trigeminal Nucleus
    UMB Dataset

    Authors
    Radi Masri
    Yadong Ji
    Alexandra Rizk
    Pamela Voulalas
    4 more author(s)...
    Description

    The prevalence of migraines in females is 3 times greater than in males. Current research has implicated the calcitonin gene-related peptide (CGRP) as a significant factor in the pathophysiology of migraines. This study investigated the sex differences associated with the expression of three protein components of the calcitonin gene-related peptide receptor: calcitonin receptor-like receptor (CLR), receptor activity-modifying protein 1 (RAMP1), and receptor component protein (RCP). The research included comparing baseline protein levels in the spinal trigeminal nucleus caudalis (SpVc) and upper cervical spinal cord between male and female rats and whether or not knock-down of the expression of RCP in the SpVc mitigates pain induced by chemical noxious stimulation of the meninges. The dataset includes western blot images (embedded within the associated article), protein expression analysis, and data associated with pain assessment and tests of facial mechanical hypersensitivity and allodynia in males versus females.

    Subject
    Migraine Disorders/physiopathology
    Receptors, Calcitonin Gene-Related Peptide/physiology
    Sex Factors
  • Thalamocortical Asynchrony in Conditions of Spinal Cord Injury Pain in Rats
    UMB Dataset

    Authors
    Radi Masri
    David A. Seminowicz
    Li Jiang
    Yadong Ji
    2 more author(s)...
    Description

    This study was undertaken to investigate the neuropathological mechanisms associated with spinal cord injury (SCI) pain. The research focused on the possible abnormal functional connectivity between the thalamus and cortex believed to be the etiology of SCI pain. A total of 14 rats were included in the research separated into 2 groups: SCI (8) and sham-operated (6). Resting-state functional magnetic resonance imaging scans were performed before surgery and postoperatively at 3, 7, 14, and 21 days. Dataset includes neuroimages and Seed-Based Correlation Analysis values.

    Subject
    Chronic Pain
    Spinal Cord Injuries
  • Nanomagnetic-Mediated Drug Delivery for the Treatment of Dental Disease
    UMB Dataset

    Authors
    Radi Masri
    Description

    This research tested the efficacy of utilizing nanoparticles, external magnetic forces, and dentinal tubules as conduits for delivering therapeutic agents to dental pulp. Moderate-size cavities were created in the dentin of rat mandibular molars and treated via the use of external magnetic forces to actively transport prednisolone-eluting magnetic nanoparticles to tooth pulp. Control groups included untreated teeth and teeth treated without magnetic pull. Expression of 4 cytokines were assessed at 2 weeks, 4 weeks, and 6 months. Additionally, the delivery system was investigated for use in actively guiding resin adhesives and the effect on shear bond strength on freshly extracted human molars. Dataset includes cytokine expression analysis and histological data.

    Subject
    Endodontics
  • MR-Guided Transcranial Focused Ultrasound Safely Enhances Interstitial Disperson of Large Polymeric Nanoparticles in the Living Brain
    UMB Dataset

    Authors
    Graeme F. Woodworth
    Victor Frenkel
    David S. Hersh
    Pavlos Anastasiadis
    7 more author(s)...
    Description

    This study tested the viability of using MRI-guided transcranial focused ultrasound (MRgFUS) to modify the interstitial space in the living brain. As a non-invasive technique, success in generating spatially controlled, non-destructive changes could lead to clinical applications such as enhancing delivery of therapeutics and altering fluid and pressure dynamics. The research involved the use of computer simulations for treatment planning and MRI acoustic radiation force impulse imaging for target validation. Additionally, Evans blue dye or nanoparticle probes were utilized to assess changes in the interstitium.This dataset includes a variety of histological, acoustic simulation, and electrophysiological images and tabular data of compressional acoustic properties of skull and brain tissue and physiochemical properties of nanoparticles.

    Subject
    Brain
    Magnetic Resonance Imaging, Interventional
    Ultrasonic Therapy
    Access Rights
    Unrestricted access
  • Approaches to Potentiated Neuroprotective Treatment in the Rodent Model of Ischemic Optic Neuropathy
    UMB Dataset

    Authors
    Zara Mehrabian
    Yan Guo
    Neil R. Miller
    Amanda D. Henderson
    2 more author(s)...
    Description

    Nonarthritic anterior ischemic optic neuropathy (NAION) commonly causes sudden optic nerve (ON)-related vision loss. The rodent NAION model (rAION) closely resembles NAION in presentation and physiological responses. Researchers hypothesized that blocking pro-inflammatory prostaglandin (PGE2) production by inhibiting monoacylglycerol lipase or cyclooxygenase activity and co-administering PGJ2 would potentiate RGC survival following ischemic neuropathy. This study identified early rAION-associated optic nerve head (ONH) inflammatory gene expression responses and the anti-inflammatory prostaglandin PGJ2’s effects on those responses. Deep sequencing was performed on vehicle- and PGJ2-treated ONHs 3d post-rAION induction. Results were compared against responses from a retinal ischemia model. Animals were treated with PGJ2 and MAGL inhibitor KML29, or PGJ2 + COX inhibitor meloxicam. RGC survival was quantified by stereology.

    Subject
    Optic Nerve Diseases
    Timeframe
    2021
    Access Rights
    Unrestricted access
  • Dopamine in the dorsal bed nucleus of stria terminalis signals Pavlovian sign-tracking and reward violations
    UMB Dataset

    Authors
    Utsav Gyawali
    David A. Martin
    Fangmiao Sun
    Yulong Li
    1 more author(s)...
    Description

    Fiber optics were implanted into the dorsal bed nucleus of the stria terminalis (dBNST) of 8-week-old male and female Sprague Dawley rats. Dopamine activity was measured using GRABda signals in the dBNST during a series of behavioral experiments. Experiments includes a Pavlovian lever autoshaping (PLA) training, a reward prediction error (RPE) probe session, and a satiety test. Rats were injected with fentanyl prior to the PLA session.

    Subject
    Receptors, Dopamine
    Access Rights
    Unrestricted access
  • Cocaine-induced neuron subtype mitochondrial dynamics through Egr3 transcriptional regulation
    UMB Dataset

    Authors
    Shannon L. Cole
    Ramesh Chandra
    Maya Harris
    Ishan Patel
    8 more author(s)...
    Description

    This dataset was generated to investigate long-lasting neurobiological adaptations to drugs of abuse. This dataset specifically supported the study of cocaine exposure impact of mitochondrial dynamics and morphology through early growth response factor 3 (Egr3) transcriptional regulation of mitochondria-related nuclear gene transcripts. Mitochondria-related nuclear genes were assayed following contingent or non-contingent cocaine exposure in rodents and were also examined in postmortem nucleus accumbens (NAc) of cocaine dependents.

    Supplemental data in tabular forms include human demographics, DNA sequencing primers, mitochondrial statistical analysis, and gene regulations.

    The following data elements were observed over a period of 7-10 days: Egr3 change in response to cocaine vs saline between rats and human postmortem issue; Egr3 binding changes in various genes promoting mitochondria in response to cocaine vs saline between rats and human postmortem issue; mRNA change in response to cocaine and saline between rats and human postmortem issue and between cocaine addict and control group; mRNA change in response to cocaine and saline between rats and human dead issue; Ribosome (the RNA binding proteins) associated mRNA changes in dopamine receptor-1 containing medium spiny neurons (D1 MSNs) and D2 MSNs in response to cocaine and saline between cocaine addict and control group.

    Subject
    Cocaine/pharmacology
    Early Growth Response Protein 3
    Mitochondrial Dynamics
    Neurons
    Transcription, Genetic/drug effects
    Access Rights
    Unrestricted access
    Approval required