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UMB Dataset

Differential nanoscale organization of excitatory synapses onto excitatory vs. inhibitory neurons

UID: 222
* Corresponding Author
Description
Primary hippocampal neuron cultures obtained from Sprague-Dawley rat embryos were infected with lentivirus expressing EGFP. Immunostaining with specific antibodies was performed on fixed neurons. Confocal and DNA-PAINT microscopy were used for imaging, and custom analysis methods were applied to analyze the acquired images. Statistical analyses were conducted using GraphPad PRISM.
Subject of Study
Subject Domain
Keywords

Access

Restrictions
Unrestricted access
Instructions
Data from this paper have been deposited in the Open Science Framework. Source code for the analyses has been made available in GitHub.
Access via OSF

Data underlying figures

Access via GitHub

Source code

Associated Publications
Equipment Used
Andor Dragonfly confocal unit
Andor Zyla4.2 sCMOS camera
Mad City Labs RM21 microscope base
Nikon Apo TIRF 60×/1.49 NA objective
Nikon Ti2 Eclipse inverted microscope
Olympus UPlanApo60x OHR/1.5 NA objective
Photometrics Prime95b sCMOS camera
Software Used
GraphPad Prism
SynQuant
Study Type
Interventional
Dataset Format(s)
XLSX
Dataset Size
5.2 MB
Grant Support