UMB Data Catalog

A project of the Health Sciences and Human Services Library

Filter by

of 1 Next >
Results Found: 2
  • UMB Dataset

    Differential nanoscale organization of excitatory synapses onto excitatory vs. inhibitory neurons

    Authors
    Poorna A. Dharmasri
    Aaron D. Levy
    Thomas A. Blanpied
    Description

    Primary hippocampal neuron cultures obtained from Sprague-Dawley rat embryos were infected with lentivirus expressing EGFP. Immunostaining with specific antibodies was performed on fixed neurons. Confocal and DNA-PAINT microscopy were used for imaging, and custom analysis methods were applied to analyze the acquired images. Statistical analyses were conducted using GraphPad PRISM.

    Subject
    Disks Large Homolog 4 Protein/metabolism
    Interneurons/physiology
    Access Rights
    Unrestricted access
  • UMB Dataset

    Single-Nucleus RNA-Seq Reveals Dysregulation of Striatal Cell Identity Due to Huntington’s Disease Mutations

    Authors
    Sonia Malaiya
    Marcia Cortes-Gutierrez
    Brian R. Herb
    Sydney R. Coffey
    5 more author(s)...
    Description

    The involvement of specific medium spiny neuro (MSN) subtype and of other striatal cell types remains poorly understood. To gain insight into cell type-specific disease processes, researchers studied the nuclear transcriptomes of 4524 cells from the striatum of a genetically precise knock-in mouse model of the Huntington's Disease (HD) mutation, HttQ175/1. Three 14-month-old and one 15-month-old male...

    Subject
    Sequence Analysis, RNA
    Timeframe
    2020
    Access Rights
    Unrestricted access