Cocaine-induced neuron subtype mitochondrial dynamics through Egr3 transcriptional regulation

Description

This dataset was generated to investigate long-lasting neurobiological adaptations to drugs of abuse. This dataset specifically supported the study of cocaine exposure impact of mitochondrial dynamics and morphology through early growth response factor 3 (Egr3) transcriptional regulation of mitochondria-related nuclear gene transcripts. Mitochondria-related nuclear genes were assayed following contingent or non-contingent cocaine exposure in rodents and were also examined in postmortem nucleus accumbens (NAc) of cocaine dependents.

Supplemental data in tabular forms include human demographics, DNA sequencing primers, mitochondrial statistical analysis, and gene regulations.

The following data elements were observed over a period of 7-10 days: Egr3 change in response to cocaine vs saline between rats and human postmortem issue; Egr3 binding changes in various genes promoting mitochondria in response to cocaine vs saline between rats and human postmortem issue; mRNA change in response to cocaine and saline between rats and human postmortem issue and between cocaine addict and control group; mRNA change in response to cocaine and saline between rats and human dead issue; Ribosome (the RNA binding proteins) associated mRNA changes in dopamine receptor-1 containing medium spiny neurons (D1 MSNs) and D2 MSNs in response to cocaine and saline between cocaine addict and control group.

Subject of Study

Human postmortem tissue

Rats (Sprague Dawley)

Subject Domain

Cocaine/pharmacology

Early Growth Response Protein 3

Mitochondrial Dynamics

Neurons

Transcription, Genetic/drug effects

Keywords

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Access Restrictions: Unrestricted access

Approval required
Access Instructions: Supplementary data and graphs can be found within the article; contact the corresponding author for all other data requests.
Associated Publications: Cole, S. L., Chandra, R., Harris, M., Patel, I., Wang, T., Kim, H., Jensen, L., Russo, S. J., Turecki, G., Gancarz-Kausch, A. M., Dietz, D. M., & Lobo, M. K. (2021). Cocaine-induced neuron subtype mitochondrial dynamics through Egr3 transcriptional regulation. Molecular brain, 14(1), 101. https://doi.org/10.1186/s13041-021-00800-y
Data Type: Biospecimens

Gene Expression Analysis

Confocal Image

Quantitative
Equipment Used: BioAnalyzer 2100
Automated electrophoresis tool for the sample quality control of biomolecules

Leica SP8 confocal microscope
Used for fluorescence imaging

Leica Vibratome
Used to cut thin slices of tissue

Thermo Scientific NanoDrop Spectrophotometer
Benchtop spectrophotometer designed for DNA, RNA, and protein quantification.
Software Used: GraphPad Prism 6.0
Used for statistical analysis

Imaris 8.2
Used for interactive microscopy image analysis
Study Type: Empirical Research
Dataset Format(s): Graph, Confocal Image
Data Collection Instruments: 33-gauge Hamilton syringe

Qiagen RNeasy MicroKit

Qiagen QIAquick PCR Purification Kit

Omega Total RNA Kit

Invitrogen Dynabeads Protein G

Invitrogen TRIzol

Quanta PerfeCTa SYBR Green FastMix

Polysciences Aqua-Poly/Mount

Grant Support: R01DA038613/NIH

NARSAD Young Investigator Grant 23621 P&S Fund/Brain and Behavior Research Foundation

UMB Data Catalog

Cocaine-induced neuron subtype mitochondrial dynamics through Egr3 transcriptional regulation
UMB Dataset

Cocaine-induced neuron subtype mitochondrial dynamics through Egr3 transcriptional regulation UMB Dataset

Cocaine-induced neuron subtype mitochondrial dynamics through Egr3 transcriptional regulation
UMB Dataset