UMB Data Catalog

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Results Found: 14
  • UMB Dataset

    Methylsulfonylmethane Increases the Alveolar Bone Density of Mandibles in Aging Female Mice

    Authors
    Hanan Aljohani
    Linda T. Senbanjo
    Mohammed Al Qranei
    Joseph P. Stains
    1 more author(s)...
    Description

    Thirty-six-week-old again female C5BL/6 mice were injected with MSM for thirteen weeks. Researchers examined the effects of MSM on bone density and development using micro-computed tomographic images, serum analyses, and immunohistochemical analyses.

    Subject
    Osteogenesis
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  • UMB Dataset

    Prion Replication Environment Defines the Fate of Prion Strain Adaptation

    Authors
    Ilia V. Baskakov
    Elizaveta Katorcha
    Nuria Gonzalez-Montalban
    Natallia Makarava
    1 more author(s)...
    Description

    The research associated with this dataset explored cross-species prion transmission and the impact of cofactor environment on strain adaptation. Utilizing Protein Misfolding Cyclic Amplification with beads (PMCAb), hamster strain 263K was propagated under normal or RNA-depleted conditions first in mouse and then hamster substrates. A variety of readaptation experiments were conducted including brain...

    Subject
    Prion Diseases
    Zoonoses
    Access Rights
    Unrestricted access
  • UMB Dataset

    Tip60- and Sirtuin 2-Regulated MARCKS Acetylation and Phosphorylation Are Required for Diabetic Embryopathy

    Authors
    Penghua Yang
    Cheng Xu
    E. Albert Reece
    Xi Chen
    5 more author(s)...
    Description

    A high glucose level associated with maternal diabetes has been identified as a risk factor for fetal neural tube defects (NTD). The failure to successfully complete the complex neurulation process is possibly related to the loss of protection for cellular homeostasis resulting in organelle stress. Mitochondrial dysfunction and endoplasmic reticulum stress in the developing endothelium have been identified...

    Subject
    Neurulation
    Pregnancy in Diabetics
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  • UMB Dataset

    Region-Specific Response of Astrocytes to Prion Infection

    Authors
    Natallia Makarava
    Jennifer Chen-Yu Chang
    Rajesh Kushwaha
    Ilia V. Baskakov
    Description

    Chronic neuroinflammation is a common pathological hallmark of neurodegenerative diseases such as Alzheimer’s, Parkinson’s, ALS, and prion disorders. Both reactive microgliosis and astrogliosis have been identified as having a role in the neuroinflammatory process. Studies of prion diseases of animals and humans have demonstrated activation and proliferation of microglia at the very early subclinical...

    Subject
    Neurodegenerative Diseases
    Prion Diseases
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    Unrestricted access
  • UMB Dataset

    Approaches to Potentiated Neuroprotective Treatment in the Rodent Model of Ischemic Optic Neuropathy

    Authors
    Zara Mehrabian
    Yan Guo
    Neil R. Miller
    Amanda D. Henderson
    2 more author(s)...
    Description

    Nonarthritic anterior ischemic optic neuropathy (NAION) commonly causes sudden optic nerve (ON)-related vision loss. The rodent NAION model (rAION) closely resembles NAION in presentation and physiological responses. Researchers hypothesized that blocking pro-inflammatory prostaglandin (PGE2) production by inhibiting monoacylglycerol lipase or cyclooxygenase activity and co-administering PGJ2 would...

    Subject
    Optic Nerve Diseases
    Timeframe
    2021
    Access Rights
    Unrestricted access
  • UMB Dataset

    Biomarkers of lesion severity in a rodent model of nonarthritic anterior ischemic optic neuropathy (rNAION)

    Authors
    Yan Guo
    Zara Mehrabian
    Mary A. Johnson
    Neil R. Miller
    3 more author(s)...
    Description

    The rodent model of nonarthritic anterior ischemic optic neuropathy (rNAION) is similar in many of its pathophysiological responses to clinical NAION. Like human NAION, there is significant variability in the severity of the lesion produced, and little is known of the parameters associated with rNAION induction severity or if pre- or early post-induction biomarkers can be identified that enable prediction...

    Subject
    Optic Nerve Diseases
    Access Rights
    Unrestricted access
  • UMB Dataset

    An optimized protocol for single nuclei isolation from clinical biopsies for RNA-seq

    Authors
    Thomas V. Rousselle
    Jennifer M. McDaniels
    Amol C. Shetty
    Elissa Bardhi
    2 more author(s)...
    Description

    Researchers present an optimized protocol for single nuclei RNA sequencing (snRNA-seq) that is fast, low, cost, and time effective due to the elimination of cell sorting and ultra-centrifugation. Both human and mouse tissue biopsies were assessed. Human kidney graft biopsies were collected from five kidney transplant recipients. Mouse needle biopsies were collected from heart, liver, kidney, spleen,...

    Subject
    High-Throughput Nucleotide Sequencing
    Access Rights
    Unrestricted access
  • UMB Dataset

    Refinement of a Live Attenuated Salmonella enterica Serovat Newport Vaccine with Improved Safety

    Description

    Non-typhoidal Salmonella (NTS) is responsible for approximately 93 million cases of gastroenteritis annually. Little research has been directed towards a development of vaccines against Salmonella serogroups O:6,7 or O:8. Researchers constructed a live attenuated serogroup O:8 vaccine, CVS 1979, by deleting guaBA, htrA, and aroA from the genome of S. Newport. Over the course of 6 weeks, 6 to 8-week-old...

    Subject
    Salmonella Vaccines
    Vaccine Development
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    Unrestricted access
  • UMB Dataset

    The optic nerve lamina region is a neural progenitor cell niche

    Authors
    Steven L. Bernstein
    Yan Guo
    Rebecca Fawcett
    Candace Kerr
    3 more author(s)...
    Description

    Tissue from the optic nerve lamina region (ONLR), were dissected from both mice and humans. Immunohistochemistry (IHC) was then performed on tissue sections, and Ki67 mitosis assays assessed cell proliferation. Cell cultivation techniques isolated and cultured cells from ONLR and optic nerve tissues. Further experiments induced cell differentiation and neurosphere formation. Transgenic animal studies...

    Subject
    Optic Nerve
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    Approval required
  • UMB Dataset

    Development of a Reproducible Swine Model of Chronic Ischemic Mitral Regurgitation: Lessons Learned

    Authors
    Chetan Pasrija
    Rachael W. Quinn
    Hani Alkhatib
    Douglas Tran
    7 more author(s)...
    Description

    Thirty-five Yorkshire cross swine underwent percutaneous myocardial infarction with ethanol ablation of the circumflex or obtuse marginal (OM) arteries. Echocardiography was performed at various intervals to assess the development of ischemic mitral regurgitation (IMR) and cardiac function. Swine that developed significant IMR were scheduled for mitral valve operations, which were performed using cardiopulmonary...

    Subject
    Mitral Valve Insufficiency
    Access Rights
    Unrestricted access