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Prion Replication Environment Defines the Fate of Prion Strain Adaptation
UMB Dataset

UID: 51

Author(s): Ilia V. Baskakov*, Elizaveta Katorcha, Nuria Gonzalez-Montalban, Natallia Makarava, Gabor G. Kovacs * Corresponding Author
Description
The research associated with this dataset explored cross-species prion transmission and the impact of cofactor environment on strain adaptation. Utilizing Protein Misfolding Cyclic Amplification with beads (PMCAb), hamster strain 263K was propagated under normal or RNA-depleted conditions first in mouse and then hamster substrates. A variety of readaptation experiments were conducted including brain analysis of hamsters inoculated with different PMCAb-derived material. Dataset includes Western blot and comparative histopathological brain images.
Subject of Study
Hamster

Golden Syrian

Subject Domain
Prion Diseases
Zoonoses
Keywords
Cofactors (Biochemistry)
Replication Environment
Strain Adaptation
Access via Article

7 embedded figures, 1 table, 9 supplementary files

Access Restrictions
Unrestricted access
Access Instructions
All relevant data are within the paper and supplementary files
Associated Publications
Katorcha E, Gonzalez-Montalban N, Makarava N, Kovacs GG, Baskakov IV. (2018). Prion replication environment defines the fate of prion adaptation. PLoS Pathogens, 14(6): e1007093.
Data Type
Histopathological Images
Western Blot Images
Software Used
AlphaView

Quantitative Analysis Software

Study Type
Empirical Research
Grant Support
R01NS045585/NIH
Other Resources
S1 Figure

Analysis of RNA content in RNA-depleted NBH (PDF)

S2 Figure

Histopathological analysis of spongiform in the brains from the 2nd passage of 263KMH, 263K(M)H or 263K(MH) (PDF)

S3 Figure

Lesion profile and PrP immunopositivity score in hamsters from the 2nd passage of 263KMH, 263K(M)H or 263K(MH) (PDF)

S4 Figure

Histopathological Analysis of Spongiform Changes Using Staining with Hematoxylin and Eosin or PrP Immunoreactivity Using SAF-84 Antibody of Aged-Matched Control Group (PDF)

S5 Figure

Analysis of GdnHCl-Induced Denaturation Profiles of PrPSc (PDF)

S6 Figure

Histopathological Analysis of Spongiform in the Brains From the 2nd and 3rd Passages of 263K MH and Animals Inoculated with 263K (PDF)

S7 Figure

Immunostaining for PrP displayed the following types of PrP deposits: Diffuse/Synaptic fine deposits, intra- and perineuronal deposits, plaques and mini-plaques in the subependymal area, small granular accumulations on the ependyma, and amorphous plaque-like deposits in the subpial and subependymal regions (PDF)

S8 Figure

Lesion profile and PrP immunopositivity score in hamsters from the 2nd and 3rd passages of 263KMH and 263K groups (PDF)

S9 Figure

Histopathological analysis of spongiform in the brains from the 3rd passage of 263K(M)H and 263K(MH) (PDF)