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An optimized protocol for single nuclei isolation from clinical biopsies for RNA-seq
UMB Dataset

UID: 209

Author(s): Thomas V. Rousselle, Jennifer M. McDaniels, Amol C. Shetty, Elissa Bardhi, Daniel G. Maluf, Valeria R. Mas* * Corresponding Author
Description
Researchers present an optimized protocol for single nuclei RNA sequencing (snRNA-seq) that is fast, low, cost, and time effective due to the elimination of cell sorting and ultra-centrifugation. Both human and mouse tissue biopsies were assessed. Human kidney graft biopsies were collected from five kidney transplant recipients. Mouse needle biopsies were collected from heart, liver, kidney, spleen, lung, duodenum, large intestine, bone marrow and brain.
Subject of Study
Subject Domain
Keywords
Access via GEO

Raw sequence data
Accession #: GSE195719

Access Restrictions
Unrestricted access
Access Instructions
The raw sequence data generated in this study were deposited in the GEO
Associated Publications
Data Type
Equipment Used
ActinGreen
BioAnalyzer 2100
Chromium Next GEM Single Cell 5สน Kit v2
Countess II FL Automated Cell Counter
DAPI
Flowmi Cell Strainer
Illumina NovaSeq 6000 Sequencing System
Mitotracker CMXros
Qubit fluorometer
Sorvall Legend Micro 21
Sorvall ST 16
Software Used
10x Genomics Cellranger DNA
Seurat
Study Type
Interventional
Dataset Format(s)
SOFT, TXT, MINiML, TAR
Data Collection Instruments
Principle component analysis
Uniform manifold approximation and projection
Dataset Size
167 Mb
Grant Support
R01DK109581/National Institute of Diabetes and Digestive and Kidney Diseases (U.S.)
R01DK122682/National Institute of Immunology, Allergy and Infectious Diseases
1U01AI170050/National Institute of Immunology, Allergy and Infectious Diseases