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Results Found: 13
  • UMB Dataset

    Culture Condition of Bone Marrow Stromal Cells Affects Quantity and Quality of the Extracellular Vesicles

    Authors
    Amanda L. Scheiber
    Cierra A. Clark
    Takashi Kaito
    Masahiro Iwamoto
    3 more author(s)...
    Description

    Extracellular vehicles (EVs) released from bone marrow stromal cells (BMSCs) have been demonstrated to play an important role in their therapeutic actions for various diseases. This study establishes a scalable and reproducible EV manufacturing processes needed to develop successful implementation of EV therapy for patients. Using human BMSCs isolated from nine healthy donors, researchers examined...

    Subject
    Cell- and Tissue-Based Therapy/methods
    Access Rights
    Unrestricted access
  • UMB Dataset

    Single-Nucleus RNA-Seq Reveals Dysregulation of Striatal Cell Identity Due to Huntington’s Disease Mutations

    Authors
    Sonia Malaiya
    Marcia Cortes-Gutierrez
    Brian R. Herb
    Sydney R. Coffey
    5 more author(s)...
    Description

    The involvement of specific medium spiny neuro (MSN) subtype and of other striatal cell types remains poorly understood. To gain insight into cell type-specific disease processes, researchers studied the nuclear transcriptomes of 4524 cells from the striatum of a genetically precise knock-in mouse model of the Huntington's Disease (HD) mutation, HttQ175/1. Three 14-month-old and one 15-month-old male...

    Subject
    Sequence Analysis, RNA
    Timeframe
    2020
    Access Rights
    Unrestricted access
  • UMB Dataset

    Genotyping Plasmodium falciparum gametocytes using amplicon deep sequencing

    Authors
    Jimmy Vareta
    Natalie A. Horstman
    Matthew Adams
    Karl B. Seydel
    4 more author(s)...
    Description

    A data set of polymorphic regions of the P. falciparum genome was mined to identify a gametocyte genotyping marker. To assess marker resolution, the number of unique haplotypes in the marker region was estimated from 205 Malawian P. falciparum whole genome sequences. Specificity of the marker for detection of mature gametocytes was evaluated using reverse transcription-polymerase chain reaction of...

    Subject
    Plasmodium falciparum/genetics
    Access Rights
    Unrestricted access
  • UMB Dataset

    Dynamics of the Gut Microbiome in Shigella-Infected Children During the First Two Years of Life

    Authors
    Esther Ndungo
    Johanna B. Holm
    Syze Gama
    Andrea G. Buchwald
    4 more author(s)...
    Description

    This dataset was used to overview the taxonomic composition of the gastrointestinal microbiomes during the first two years of life with the intent to better understand the effects of the invasive pathogen, Shigella. Data was collected through a two year longitudinal study of a mother-infant cohort in Malawi, using 16S rRNA amplicon sequencing to characterize the gastrointestinal microbiota. Rectal...

    Subject
    Gastrointestinal Microbiome
    Genomics
    Shigella
    Access Rights
    Unrestricted access
  • UMB Dataset

    Neuropeptide identification using eyestalk-RNAseq of red deep-sea crab, Chaceon quenquedens

    Authors
    Shadaesha Green
    Tsvetan Bachvaroff
    J. Sook Chung
    Description

    This study aimed to understand the role of eyestalk neuropeptides in vitellogenesis. Molt-inhibiting hormone (MIH) and crustacean hyperglycemic hormone (CHH) cDNA sequences were first isolated from the eyestalk ganglia of C. quinquedens using traditional cloning methods and their expression was quantified in the eyestalk ganglia at various ovarian stages. De novo transcriptome assembly of the eyestalk...

    Subject
    Brachyura/genetics
    Invertebrate Hormones/genetics
    Neuropeptides
    Access Rights
    Unrestricted access
  • UMB Dataset

    Identifying enhancer properties associated with genetic risk for complex traits using regulome-wide association studies

    Authors
    Alex M. Casella
    Carlo Colantuoni
    Seth A. Ament
    Description

    This study introduces a novel application of the MAGMA (Multi-marker Analysis of GenoMic Annotation) software tool that enables testing for associations between enhancer attributes and risk to determine the enhancer characteristics that are associated with risk for schizophrenia. The study uses the RWAS (Regulome Wide Association Study) framework to first collect enhancer annotations in a tissue...

    Subject
    Genome-Wide Association Study
    Multifactorial Inheritance
    Access Rights
    Unrestricted access
  • UMB Dataset

    Strain-specific alterations in gut microbiome and host immune responses elicited by tolerogenic Bifidobacterium pseudolongum

    Authors
    Bing Ma
    Samuel J. Gavzy
    Vikas Saxena
    Yang Song
    12 more author(s)...
    Description

    To determine strain-specific driving mechanisms of B. pseudolongum UMB-MBP-01, researchers compared it to porcine tropic strain B. pseudolongum ATCC25526 using cell culture and in vivo experimentation and comparative genomic approaches. The data demonstrates that these two strains possess distinct genetic repertoires in carbohydrate assimilation, differential activation signatures and cytokine responses...

    Subject
    Immunity
    Access Rights
    Unrestricted access
  • UMB Dataset

    Small molecules inhibitors of the heterogeneous ribonuclear protein A18 (hnRNP A18): a regulator of protein translation and an immune checkpoint

    Authors
    Eduardo Solano-Gonzalez
    Katherine M. Coburn
    Wenbo Yu
    Gerald M. Wilson
    6 more author(s)...
    Description

    Researchers utilized SILCS-based techniques to map the functional group requirements of hnRNP A18, aiming to create a pharmacophore for virtual screening. Data was obtained using the crystal structure of the hnRNP A18 RRM domain and various software tools. These simulations generated 3D functional group probability distributions of organic solutes, forming the basis for pharmacophore models. Virtual...

    Subject
    RNA-Binding Proteins / chemistry
    RNA-Binding Proteins/antagonists & inhibitors
    Access Rights
    Unrestricted access
  • UMB Dataset

    Relative contributions of various endogenous and exogenous factors to the mosquito microbiota

    Authors
    Haikel N. Bogale
    Matthew V. Cannon
    Kalil Keita
    Denka Camara
    10 more author(s)...
    Description

    Researchers used a high-throughput sequencing-based assay to characterize the bacterial composition and diversity of 665 individual field-caught mosquitoes, as well as their species, genotype at an insecticide resistance locus, blood-meal composition, and the eukaryotic parasites and viruses they carry. This data was used to rigorously estimate the individual effect of each parameter on the bacterial...

    Subject
    Pest Control, Biological
    Access Rights
    Unrestricted access
  • UMB Dataset

    The heme-responsive PrrH sRNA regulates Pseudomonas aeruginosa pyochelin gene expression

    Authors
    Tra-My Hoang
    Weiliang Huang
    Jonathan Gans
    Jacob Weiner
    5 more author(s)...
    Description

    Bacterial strains, including Pseudomonas aeruginosa, were cultured in media supplemented with iron or heme under different growth conditions, including shaking and static cultures, and exposed to different light conditions (ambient, infrared, dark). For RNA isolation and analysis, cultures were treated with RNA later and processed for quantitative real-time PCR, northern blot analyses, RNA sequencing...

    Subject
    Bacterial Proteins/metabolism
    Access Rights
    Unrestricted access