UMB Dataset
Strain-specific alterations in gut microbiome and host immune responses elicited by tolerogenic Bifidobacterium pseudolongum
UID: 212
- Description
- To determine strain-specific driving mechanisms of B. pseudolongum UMB-MBP-01, researchers compared it to porcine tropic strain B. pseudolongum ATCC25526 using cell culture and in vivo experimentation and comparative genomic approaches. The data demonstrates that these two strains possess distinct genetic repertoires in carbohydrate assimilation, differential activation signatures and cytokine responses signatures in innate immune cells, and differential effects on lymph node morphology with unique local and systemic leukocyte distribution.
Access
- Restrictions
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Unrestricted access
- Instructions
- The assembly of the genomes, metagenome, and transcriptome sequences were submitted to GenBank. R codes and input dataset used to generate the heatmap was deposited in GitHub. Raw data files available to download.
TruSeq Stranded mRNA
MUSCLE
Observational
Markov Cluster Algorithm
Hidden Markov model
Rao score test
Linear discriminant analysis Effect Size
Kruskal-Wallis
Latent Dirichlet Allocation model
Canonical Correspondence Analysis
polymerase chain reaction assay
Over-representation analyses
- Grant Support
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1U01AI170050/National Institute of Immunology, Allergy and Infectious DiseasesT32AI95190-10/National Institute of Allergy and Infectious Diseases (U.S.)