Culture Condition of Bone Marrow Stromal Cells Affects Quantity and Quality of the Extracellular Vesicles

UMB Dataset

Access via GEO

Sequencing data
Accession #: GSE185942

AH-629 swinging bucket rotor

Facilitate large volume particle isolations.

BD LSR II Flow Cytometer

Benchtop high-performance cell sorter with multiple lazer, detector and fluoroscrome options. Allows detection of 12 parameters (10 color conjugates, forward and sideward scatter).

BioAnalyzer 2100

Automated electrophoresis tool for the sample quality control of biomolecules

Bioanalyzer High Sensitivity DNA Kit

For the separation, sizing and quantification of dsDNA samples of limited abundance and sized from 50 - 7000 bp.

CellBIND 12-well plate

For sample preparation, high-throughput screening, and for the investigation of biological properties

CellBIND culture dish

Disposable or reusable shallow containers specifically designed to support the growth and propagation of cells in culture.

Illumina NovaSeq 6000 Sequencing System

Performs whole-genome sequencing

mirVana miRNA Isolation Kit

Uses a rapid procedure to isolate small RNAs from tissue and cells using an efficient glass fiber filter (GFF)-based method.

Nanosight NS300 Analyzer

Rapid, automated analysis of the size distribution and concentration of all types of nanoparticles

QIAseq miRNA Library Kit

For sequencing prep reactions: 3’ ligation, 5’ ligation, reverse-transcription, cDNA cleanup, library amplification and library cleanup reagents; quality control primers.

Sorvall Discovery 90SE

Uses centrifugal force to separate the contents of a sample based on their density.

StemPro Adipogenesis Differentiation Kit

For the adipogenic differentiation of mesenchymal stem cells (MSCs) in tissue culture vessels.

StemPro Chrondrogenesis Differentiation Kit

For the chondrogenic differentiation of mesenchymal stem cells (MSCs) in tissue-culture vessels.

StemPro Osteogenesis Differentiation Kit

For the osteogenic differentiation of mesenchymal stem cells (MSCs) in tissue culture vessels.

DESeq

Differential gene expression analysis based on the negative binomial distribution

FlowJo

Software for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.

GraphPad Prism

A tool for analyzing and visualizing complex biological data, particularly in the fields of genomics and proteomics.

Illumina bcl2fastq

Conversion software that both demultiplexes data and converts BCL files generated by Illumina sequencing systems to standard FASTQ file formats for downstream analysis.

miRNA target filter

Enables prioritization of experimentally validated and predicted mRNA targets.

NanoSight NTA

Utilizes the properties of both light scattering and Brownian motion in order to obtain the nanoparticle size distribution of samples in liquid suspension.

QIAGEN GeneGlobe Data Analysis Center

Web based portal using web based tools for analyzing real-time qPCR or next-generation sequencing data.

QIAGEN IPA

Quickly visualize and understand complex ‘omics data and perform insightful data analysis and interpretation by placing your experimental results within the context of biological systems.

Interventional

Benjamini–Hochberg method

unpaired two-tailed t-test

One-Way ANOVA

Two-Way ANOVA

Tukey's multiple comparison test