UMB Data Catalog
A project of the Health Sciences and Human Services LibraryNeuropeptide identification using eyestalk-RNAseq of red deep-sea crab, Chaceon quenquedens
UMB Dataset
UID: 184
- Description
- This study aimed to understand the role of eyestalk neuropeptides in vitellogenesis. Molt-inhibiting hormone (MIH) and crustacean hyperglycemic hormone (CHH) cDNA sequences were first isolated from the eyestalk ganglia of C. quinquedens using traditional cloning methods and their expression was quantified in the eyestalk ganglia at various ovarian stages. De novo transcriptome assembly of the eyestalk ganglia was conducted to determine if transcripts of other neuropeptides differ by ovarian stages. Samples were collected from the mid-Atlantic Bight region of the United States off the coast of Virginia up to Massachusetts between 2013 and 2015.
- Timeframe
- 2013 - 2015
- Geographic Coverage
-
Mid-Atlantic Bight
-
Access via NCBI SRA
RNA sequencing datasets under the BioProject PRJNA765736
Accession #: PRJNA765736Access via Appendix A.Supplementary data from the article.
- Access Restrictions
-
Unrestricted access
- Access Instructions
- The RNA sequencing data sets have been submitted to NCBI Sequence Read Archive (SRA) under the BioProject PRJNA765736. Supplementary data to this article can be found online at https://doi.org/10.1016/j.ygcen.2022.114128
- Associated Publications
- Data Type
- Equipment Used
-
Jouan SpeedVac
- Software Used
-
RegRNA
- Study Type
-
Empirical Research
- Dataset Format(s)
- Plain Text, FASTQ, DOCX
- Dataset Size
- 8169 Mb
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