This study aimed to understand the role of eyestalk neuropeptides in vitellogenesis. Molt-inhibiting hormone (MIH) and crustacean hyperglycemic hormone (CHH) cDNA sequences were first isolated from the eyestalk ganglia of C. quinquedens using traditional cloning methods and their expression was quantified in the eyestalk ganglia at various ovarian stages. De novo transcriptome assembly of the eyestalk ganglia was conducted to determine if transcripts of other neuropeptides differ by ovarian stages. Samples were collected from the mid-Atlantic Bight region of the United States off the coast of Virginia up to Massachusetts between 2013 and 2015.