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  • UMB Dataset

    An optimized protocol for single nuclei isolation from clinical biopsies for RNA-seq

    Authors
    Thomas V. Rousselle
    Jennifer M. McDaniels
    Amol C. Shetty
    Elissa Bardhi
    2 more author(s)...
    Description

    Researchers present an optimized protocol for single nuclei RNA sequencing (snRNA-seq) that is fast, low, cost, and time effective due to the elimination of cell sorting and ultra-centrifugation. Both human and mouse tissue biopsies were assessed. Human kidney graft biopsies were collected from five kidney transplant recipients. Mouse needle biopsies were collected from heart, liver, kidney, spleen,...

    Subject
    High-Throughput Nucleotide Sequencing
    Access Rights
    Unrestricted access