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  • Seasonal changes in the expression of insulinlike androgenic hormone (IAG) in the androgenic gland of the Jonah crab, Cancer borealis
    UMB Dataset

    Authors
    Amanda Lawrence
    Shadaesha Green
    Tao Wang
    Tsvetan Bachvaroff
    1 more author(s)...
    Description

    This study aimed to identify the insulin-like androgenic gland factor (IAG) in adult male Jonah crabs as it plays an essential role in sexual maturity. The study also aimed to examine if seasonal changes influence IAG expression in males. The study used the androgenic gland transcriptome to test if eyestalk neuropeptides regulate IAG levels via an endocrine axis between the two endocrine tissues as established in other crustaceans. Understanding the size related-sexual maturity and the seasonal changes in Jonah crab male reproductive activity is critical for sustainable fishery management. The full-length CabIAG sequence is 928 nucleotides long, encoding a 151 amino acid deduced sequence. This gene expression was exclusive in male C. borealis AG. Partial CoDing Sequence (CDS) of cancer borealis Na/K-ATPase alpha subunit mRNA was deposited in the GenBank.

    Subject
    Brachyura
    Sexual Maturation
    Transcriptome
    Geographic Coverage
    United States
    Access Rights
    Unrestricted access

  • Neuropeptide identification using eyestalk-RNAseq of red deep-sea crab, Chaceon quenquedens
    UMB Dataset

    Authors
    Shadaesha Green
    Tsvetan Bachvaroff
    J. Sook Chung
    Description

    This study aimed to understand the role of eyestalk neuropeptides in vitellogenesis. Molt-inhibiting hormone (MIH) and crustacean hyperglycemic hormone (CHH) cDNA sequences were first isolated from the eyestalk ganglia of C. quinquedens using traditional cloning methods and their expression was quantified in the eyestalk ganglia at various ovarian stages. De novo transcriptome assembly of the eyestalk ganglia was conducted to determine if transcripts of other neuropeptides differ by ovarian stages. Samples were collected from the mid-Atlantic Bight region of the United States off the coast of Virginia up to Massachusetts between 2013 and 2015.

    Subject
    Brachyura/genetics
    Invertebrate Hormones/genetics
    Neuropeptides
    Geographic Coverage
    United States
    Access Rights
    Unrestricted access

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