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  • Staphylococcus aureus strain MRSA1, whole genome shotgun sequencing project
    UMB Dataset

    Alternate Title(s)
    Examination of 388 Staphylococcus aureus Isolates from Intensive Care Unit Patients
    Authors
    Timileyin Adedrian
    Stephanie Hitchcock
    Lyndsay M. O’Hara
    Jane M. Michalski
    6 more author(s)...
    Description

    The dataset contains data pertaining to the examination of draft genomes of 388 methicillin-resistant Staphylococcus aureus isolates obtained from intensive care unit patients at three geographically distributed hospitals. The purpose of the study was to determine genomic diversity associated with potential health care worker-associated transmission. Relevant statistics, including linked GenBank and SRA accession numbers for each genome assembly, are included in Table 1 of the associated publication.

    Subject
    Disease Transmission, Infectious
    Intensive Care Units
    Methicillin-Resistant Staphylococcus aureus
    Whole Genome Sequencing
    Geographic Coverage
    Baltimore (Md.)
    United States
    Access Rights
    Unrestricted access

  • Seasonal changes in the expression of insulinlike androgenic hormone (IAG) in the androgenic gland of the Jonah crab, Cancer borealis
    UMB Dataset

    Authors
    Amanda Lawrence
    Shadaesha Green
    Tao Wang
    Tsvetan Bachvaroff
    1 more author(s)...
    Description

    This study aimed to identify the insulin-like androgenic gland factor (IAG) in adult male Jonah crabs as it plays an essential role in sexual maturity. The study also aimed to examine if seasonal changes influence IAG expression in males. The study used the androgenic gland transcriptome to test if eyestalk neuropeptides regulate IAG levels via an endocrine axis between the two endocrine tissues as established in other crustaceans. Understanding the size related-sexual maturity and the seasonal changes in Jonah crab male reproductive activity is critical for sustainable fishery management. The full-length CabIAG sequence is 928 nucleotides long, encoding a 151 amino acid deduced sequence. This gene expression was exclusive in male C. borealis AG. Partial CoDing Sequence (CDS) of cancer borealis Na/K-ATPase alpha subunit mRNA was deposited in the GenBank.

    Subject
    Brachyura
    Sexual Maturation
    Transcriptome
    Geographic Coverage
    United States
    Access Rights
    Unrestricted access

  • An optimized protocol for single nuclei isolation from clinical biopsies for RNA-seq
    UMB Dataset

    Authors
    Thomas V. Rousselle
    Jennifer M. McDaniels
    Amol C. Shetty
    Elissa Bardhi
    2 more author(s)...
    Description

    Researchers present an optimized protocol for single nuclei RNA sequencing (snRNA-seq) that is fast, low, cost, and time effective due to the elimination of cell sorting and ultra-centrifugation. Both human and mouse tissue biopsies were assessed. Human kidney graft biopsies were collected from five kidney transplant recipients. Mouse needle biopsies were collected from heart, liver, kidney, spleen, lung, duodenum, large intestine, bone marrow and brain.

    Subject
    High-Throughput Nucleotide Sequencing
    Access Rights
    Unrestricted access