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  • Region-Specific Response of Astrocytes to Prion Infection
    UMB Dataset

    Authors
    Natallia Makarava
    Jennifer Chen-Yu Chang
    Rajesh Kushwaha
    Ilia V. Baskakov
    Description

    Chronic neuroinflammation is a common pathological hallmark of neurodegenerative diseases such as Alzheimer’s, Parkinson’s, ALS, and prion disorders. Both reactive microgliosis and astrogliosis have been identified as having a role in the neuroinflammatory process. Studies of prion diseases of animals and humans have demonstrated activation and proliferation of microglia at the very early subclinical stages. Less is known about the role of reactive astrocytes. This research investigated the changes in normal functions of astrocytes over the course of chronic neuroinflammation including their region-specific responses in the brain. Studies of brain tissue of mice infected with 22L mouse-adapted prion strain include histological, immunofluorescence, and qRT-PCR. Prion deposition and reactive microgliosis and astrogliosis were examined across regions of the whole brain with particular focus on the thalamus and hippocampus.

    Subject
    Neurodegenerative Diseases
    Prion Diseases
    Access Rights
    Unrestricted access

  • Cocaine-induced neuron subtype mitochondrial dynamics through Egr3 transcriptional regulation
    UMB Dataset

    Authors
    Shannon L. Cole
    Ramesh Chandra
    Maya Harris
    Ishan Patel
    8 more author(s)...
    Description

    This dataset was generated to investigate long-lasting neurobiological adaptations to drugs of abuse. This dataset specifically supported the study of cocaine exposure impact of mitochondrial dynamics and morphology through early growth response factor 3 (Egr3) transcriptional regulation of mitochondria-related nuclear gene transcripts. Mitochondria-related nuclear genes were assayed following contingent or non-contingent cocaine exposure in rodents and were also examined in postmortem nucleus accumbens (NAc) of cocaine dependents.

    Supplemental data in tabular forms include human demographics, DNA sequencing primers, mitochondrial statistical analysis, and gene regulations.

    The following data elements were observed over a period of 7-10 days: Egr3 change in response to cocaine vs saline between rats and human postmortem issue; Egr3 binding changes in various genes promoting mitochondria in response to cocaine vs saline between rats and human postmortem issue; mRNA change in response to cocaine and saline between rats and human postmortem issue and between cocaine addict and control group; mRNA change in response to cocaine and saline between rats and human dead issue; Ribosome (the RNA binding proteins) associated mRNA changes in dopamine receptor-1 containing medium spiny neurons (D1 MSNs) and D2 MSNs in response to cocaine and saline between cocaine addict and control group.

    Subject
    Cocaine/pharmacology
    Early Growth Response Protein 3
    Mitochondrial Dynamics
    Neurons
    Transcription, Genetic/drug effects
    Access Rights
    Unrestricted access
    Approval required

  • Mutations in Diphosphoinositol-Pentakisphosphate Kinase PPIP5K2 are associated with hearing loss in human and mouse
    UMB Dataset

    Authors
    Saima Riazuddin
    Rizwan Yousaf
    Chunfang Gu
    Zubair M. Ahmed
    4 more author(s)...
    Description

    Genetically complex non-syndromic recessively inherited hearing loss (NSRHL) comprises approximately 75% of hereditary deafness. This study investigated NSRHL in two large consanguineous Pakistani families. Exome sequencing coupled with homozygosity mapping was used to identify a missense variant in PPIP5K2 gene associated with nonsyndromic, prelingual sensorineural deafness. Biochemical analysis was performed to compare wild type human PPIP5K2 with the variant. Additional research was conducted on mouse mutants to observe the effects on the outer hair cells and hearing thresholds. This dataset includes genetic, hearing function, gene expression, and histological data and images.

    Subject
    Hearing Loss
    Point Mutation
    Geographic Coverage
    International
    Access Rights
    Unrestricted access