An institutional-based cross-sectional study was conducted to assess the prevalence of self-reported symptoms of depression, anxiety, and stress during the COVID-19 pandemic among 846 healthcare workers (HCWs) in central Ethiopia. From June 25, 2020, to July 25, 2020, an online survey was administered to collect self-reported data using the Depression, Anxiety, and Stress Scale (DASS-21). Data were cleaned, coded, and analysed using SPSS Version 23. A multivariable logistic regression analysis was done to identify the associated factors for mental health outcomes at a p-value of less than 0.05.