The dataset includes information on patients aged 18 or older treated for acute promyelocytic leukemia (APL) at the University of Maryland Medical Center and Johns Hopkins Hospital between January 2000 and May 2020. It encompasses data on FLT3-ITD mutations, including allelic burden and insertion length estimation. Patient demographics, laboratory results, treatment regimens, and outcomes were recorded. Statistical analyses were performed using Stata, including chi-square tests, t-tests, ANOVA, correlations, logistic and linear regression, Kaplan–Meier survival analysis, and Cox regression to assess associations and compare survival among patient groups.