This study aimed to identify the insulin-like androgenic gland factor (IAG) in adult male Jonah crabs as it plays an essential role in sexual maturity. The study also aimed to examine if seasonal changes influence IAG expression in males. The study used the androgenic gland transcriptome to test if eyestalk neuropeptides regulate IAG levels via an endocrine axis between the two endocrine tissues as established in other crustaceans. Understanding the size related-sexual maturity and the seasonal changes in Jonah crab male reproductive activity is critical for sustainable fishery management. The full-length CabIAG sequence is 928 nucleotides long, encoding a 151 amino acid deduced sequence. This gene expression was exclusive in male C. borealis AG. Partial CoDing Sequence (CDS) of cancer borealis Na/K-ATPase alpha subunit mRNA was deposited in the GenBank.