UMB Data Catalog
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Genotyping Plasmodium falciparum gametocytes using amplicon deep sequencing
UMB Dataset- Authors
- Jimmy VaretaNatalie A. HorstmanMatthew AdamsKarl B. Seydel4 more author(s)...
- Description
A data set of polymorphic regions of the P. falciparum genome was mined to identify a gametocyte genotyping marker. To assess marker resolution, the number of unique haplotypes in the marker region was estimated from 205 Malawian P. falciparum whole genome sequences. Specificity of the marker for detection of mature gametocytes was evaluated using reverse transcription-polymerase chain reaction of RNA extracted from NF54 mature gametocytes and rings from a non-gametocyte-producing strain of P. falciparum. Amplicon deep sequencing was performed on experimental mixtures of mature gametocytes from two distinct parasite clones, as well as gametocyte-positive P. falciparum field isolates to evaluate the quantitative ability and determine the limit of detection of the genotyping approach.
- Subject
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Plasmodium falciparum/genetics
- Access Rights
- Unrestricted access