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Genomic and Transcriptomic Survey of Mucormycosis-Causing Fungi
UMB Dataset

UID: 50

Author(s): Vincent M. Bruno*, Marcus C. Chibucos, Sean Daugherty, Joshua Orvis, Amol C. Shetty, Jonathan Crabtree, Tracy H. Hazen, Priti Kumari, Timothy D. O'Connor, David A. Rasko, Claire M. Fraser * Corresponding Author
Description
Mucormycosis is a life-threatening disease especially to individuals who are immunosuppressed or have chronic health conditions. Several genera within the fungal order Mucorales have been identified as causative agents. This dataset is the result of research elucidating the population genetics, phylogeny, and pathogenicity of the infectious fungi. Included are de novo genome sequences and annotations for 27 isolates of 15 species of the following genera: Apophysomyces, Cokeromyces, Cunninghamella, Mucor, Rhizomucor, Rhizopus, Saksenaea, Syncephalastrum, and Umbelopsis. With the inclusion of previously published genomes of additional strains, comparative analysis was conducted on a total of 41. To investigate Mucorales fungi-host cell responses, RNA sequencing was performed using human airway epithelial cells infected with Rhizopus delemar or Rhizopus oryzae. All sequences are available in the NCBI SRA database with additional data provided via supplementary files.
Subject of Study
Subject Domain
Keywords
Access Restrictions
Unrestricted access
Access Instructions
Enter relevant BioProject number(s) available in Supplement Data 1 (Excel file with isolate information) in SRA search box; RNA sequences are accessible using the accession code: SRP077731
Associated Publications
Data Type
Equipment Used
Illumina HiSeq 2000 System
Study Type
Empirical Research
Grant Support
HHSN272200900009C/NIH
Other Resources
Supplementary Figures

PDF with supplementary figures 1-5

Supplementary Data 1

Excel file with isolate information

Supplementary Data 2

Word document with sequencing, assembly, and gene summary statistics

Supplementary Data 3

Excel file with top 20 MEME motifs identified

Supplementary Data 4

Excel file with genes only present in R. delemar and R. oryzae as described in Figure 4

Supplementary Data 5

Excel file with most highly expressed genes in R. delemar (99-880) genome

Supplementary Data 6

Excel file with list of differentially expressed genes in R. delemar 99-880

Supplementary Data 7

Excel file of list with differentially expressed genes in R. oryzae 99-892

Supplementary Data 8

Excel file with differentially expressed host genes

Supplementary Data 9

Excel file with upstream regulator analysis of host genes' expression