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Comparison of In Vivo Expression Levels of IL-4 and IL-4d2 in Mouse Lung Tissue
UMB Dataset

Alternate Titles(s): Comparison of In Vivo Expression Levels of IL-4 and IL-4 delta2 in Mouse Lung Tissue

UID: 13

Author(s): Sergei P. Atamas* * Corresponding Author

Description
The primary goal of this project was to compare in vivo gene expression levels of the cytokine, IL-4, and its natural splice variant, IL-4δ2, in the lungs of mice. Intratracheal replication-deficient adenovirus-mediated gene delivery of mouse IL-4 or IL-4δ2 was utilized to create three mice overexpressing IL-4 and three overexpressing IL-4δ2 in their lungs. An additional three mice were similarly infected with control AdV-NULL virus not encoding a cytokine. 14 days postinfection, lung tissue was homogenized, total RNA extracted, and results analyzed. The data demonstrate that IL-4 and its splice variant differentially affect global gene expression which has implications for the use of targeted therapy for various diseases. The dataset consists of 9 microarray data tables corresponding to each of the samples. Additionally, a supplementary TAR file of the raw data is also available to download.
Timeframe
2010 - 2010
Subject of Study
Subject Domain
Keywords
Access Restrictions
Free to All
Access Instructions
Available from the NCBI GEO site
Associated Publications
Data Type
Microarray Data
Equipment Used
Affymetrix Mouse Genome 430 2.0 Array
Software Used
GeneMaths XT
Statistica
Study Type
Empirical Research
Dataset Format(s)
Data Tables
Dataset Size
13 Mbytes
Grant Support
Veterans Administration Merit Review Award/Veterans Administration
Arthritis Foundation Review Award/Arthritis Foundation