Denture stomatitis (DS) is a chronic oral infection prevalent in denture wearers. In order to study the disease and evaluate therapeutic strategies, an intraoral dental appliance for use in a rat animal model, with universal fit, was created in the 3Shape Dental Design System. This code file was then generated for fabricating the device via 3D printing. STL format is supported by any software packages used for computer-aided design (CAD), 3D printing, rapid prototyping, and computer-aided manufacturing (CAM).