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Dopamine in the dorsal bed nucleus of stria terminalis signals Pavlovian sign-tracking and reward violations
UMB Dataset

UID: 213

Author(s): Utsav Gyawali*, David A. Martin, Fangmiao Sun, Yulong Li, Donna Calu* * Corresponding Author
Description
Fiber optics were implanted into the dorsal bed nucleus of the stria terminalis (dBNST) of 8-week-old male and female Sprague Dawley rats. Dopamine activity was measured using GRABda signals in the dBNST during a series of behavioral experiments. Experiments includes a Pavlovian lever autoshaping (PLA) training, a reward prediction error (RPE) probe session, and a satiety test. Rats were injected with fentanyl prior to the PLA session.
Subject of Study
Rats (Sprague Dawley)

Sprague Dawley

Subject Domain
Receptors, Dopamine
Keywords
Cortical Excitability
Fiber Optic Technology
Reward
Access via Zenodo

Spreadsheets
Accession #: 10.5281/ zenodo.7947009

Access Restrictions
Unrestricted access
Access Instructions
The data used in this manuscript are available on Zenodo.
Associated Publications
Gyawali U, Martin DA, Sun F, Li Y, Calu D. Dopamine in the dorsal bed nucleus of stria terminalis signals Pavlovian sign-tracking and reward violations. eLife. 2023 May 26; 12:e81980.
Data Type
Behavioral
Equipment Used
DenMat Cement Catalyst

A self-adhesive, dual-cure, easy-to-use, resin-modified glass ionomer.

DenMat Dental Cement

A self-adhesive, dual-cure, easy-to-use, resin-modified glass ionomer.

Doric Fiber optic patchcord

Used for fiber photometry. A single strand of optical fiber surrounded by a buffer and protective jacket with connectors at both ends.

Doric Fluorescence Minicube

Used for dual excitation band fiber photometry measurement.

Leica Microsystems cryostat

Used to maintain low cryogenic temperatures of samples or devices mounted within the cryostat.

Med Associates sound-attenuating cabinets

Foam-lined soundproof cabinets.

Metabond catalyst

Chemically bonds amalgam to enamel, old amalgam and dentin.

Metabond powder

Adhesive resin cement that bonds to dentin, enamel metals, and resins.

Metabond quick base

A 4-META-based, noneugenol, self-cured adhesive resin cement designed to provide ultra-high bond strength.

Newport photoreceiver

Measurement device for optical waveforms.

Test Diet sucrose pellets

Purified ingredient rodent tablet, protein: 20.6%, fat: 12.7%, carbohydrate: 66.7%

ThorLabs fiber optical cannula

Deliver light from an LED or laser light source to optically sensitive cells within a specimen.

ThorLabs LED Driver

"LED Driver capable of driving high-power four- wavelength LED sources simultaneously with a current range between 0 and 1000 mA"

ThorLabs LEDs

Designed for microscopy, illumination, and measurement applications.

Vectashield mounting medium

A stable formula for preserving fluorescence.

Software Used
GraphPad Prism

A tool for analyzing and visualizing complex biological data, particularly in the fields of genomics and proteomics.

IBM SPSS Statistics, Version 21
MATLAB

A high-level programming language and environment for numerical computing and visualization.

MED PC

Customizable software program for behavioral testing. Users logically order simple text commands to direct experimental work flow and data collection, providing control of chamber components, stimuli, reinforcement mechanisms, data variables, and arrays. Standard pre-written procedures and custom coding solutions are available for purchase.

Microsoft Excel

Spreadsheet software

Synapse software suite

Streamlined tools for neural recordings, such as filters, spike sorting, and detection of evoked responses – in addition to calcium imaging and behavioral control.

Study Type
Interventional
Dataset Format(s)
XLSX
Data Collection Instruments
Pavlovian Conditioned Approach score
Pavlovian lever autoshaping
Reward prediction error
chow satiety test
pellet satiety test
Analysis of variance
Dataset Size
217.62 MB