Description: This study investigates multiple vaccine strategies to enhance immunogenicity and protection against SARS-CoV-2 in aged mice. The study aims to determine the effect of a booster dose, with an emphasis on older age. Female three and eleven-month-old BALB/c mice were studied over the course of 38 weeks. Mice received BNT162b2 SARS-CoV-2 spike mRNA vaccine series, primary vaccination series, and mock Phosphate-buffered saline (PBS) injections. Single mouse experiments aimed to include 20 mice per group. Sample size and age criteria were chosen empirically based on the results of previous studies. Mice were randomly assigned to different treatment groups.
Local Experts: Matthew B. Frieman

Subject of Study

Subject Domain

Subject Sex

Female

Keywords

Access via Supplementary Data 1
Access Restrictions: Unrestricted access
Access Instructions: All data supporting the findings of this study are available within the supplementary information files (Supplementary Data 1). The data corresponds to the figures that are in the article.
Associated Publications: Nanishi, E., McGrath, M. E., O'Meara, T. R., Barman, S., Yu, J., Wan, H., Dillen, C. A., Menon, M., Seo, H. S., Song, K., Xu, A. Z., Sebastian, L., Brook, B., Bosco, A. N., Borriello, F., Ernst, R. K., Barouch, D. H., Dhe-Paganon, S., Levy, O., Frieman, M. B., & Dowling, D. J. (2022). mRNA booster vaccination protects aged mice against the SARS-CoV-2 Omicron variant. Communications biology, 5(1), 790. https://doi.org/10.1038/s42003-022-03765-3
Data Type: Biospecimens
Equipment Used: BD LSR II Flow Cytometer
Benchtop high-performance cell sorter with multiple lazer, detector and fluoroscrome options. Allows detection of 12 parameters (10 color conjugates, forward and sideward scatter).

Bead ruptor (Omni International Inc.)
Used for grinding, lysing, and homogenizing biological samples prior to molecular extraction.

Costar 96-well cell culture multiple well plate, flat bottom, with lid
Used for sample preparation.

UltraPure agarose (Invitrogen)
Used for analysis and recovery of DNA and RNA.
Software Used: FlowJo v.10.8.1
Single-cell flow cytometry analysis.
Study Type: Interventional

Longitudinal
Dataset Format(s): XLSX
Dataset Size: 50 KB

Grant Support: HHSN272201400052C/United States. National Institute of Allergy and Infectious Diseases

75N93019C00044/United States. National Institute of Allergy and Infectious Diseases

HHSN272201800047C/United States. National Institute of Allergy and Infectious Diseases

1R21AI137932-01A1/National Institutes of Health (U.S.)

ASPR-20-01495/Biomedical Advanced Research and Development Authority (U.S.)

UMB Data Catalog

mRNA booster vaccination protects aged mice against the SARS-CoV-2 Omicron variant
UMB Dataset